Transform One Syringe into 600 ml/cc!
Unlock the potential of your mycology projects with our Liquid Culture Expander Kit! This innovative solution allows you to effortlessly convert a single 10ml culture into an impressive 600ml of liquid culture. Never use spore syringes for this product. These Jars come ready to use and are sterilized in a mushroom grow bag for 30 minutes at 15 PSI. They require no lid changing and are ready to use! Some liquid may form be at the bottom of the bag as this is natural from sterilization. All Contents are 100% sterile.
Designed for Optimal Growth
Our specially formulated liquid culture expansion kit is crafted by experienced mycologists to promote rapid mycelial growth in a sterile, nutrient-rich environment. Within just 7 to 14 days, you’ll have a vibrant liquid culture ready for use, which can be conveniently stored at room temperature up to 1 month after fully colonized, or in a a refrigerator for up to 4 months!
Liquid Culture Expansion Kit Instructions
Materials Needed:
- Liquid culture expansion kit (jar with sterile liquid culture)
- Verified clean liquid culture
- Luer lock syringes to pull your culture when ready
Instructions:
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Observation Period:
- Store the liquid culture kit at room temperature for 7-10 days before use. During this time, observe the jar for signs of growth or contamination.
- It is essential to ensure that there is no evident growth. The solution is distilled water and light corn syrup
- During this time Verify your liquid culture being used is clean on agar
- After both "mother" liquid culture and jar have been verified sterile, continue on to step 2
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Inoculating the Liquid Culture:
- Wipe injection port down with alcohol pad
- Using the mother liquid culture syringe, inject 1-10 mL of the liquid culture into the jar.
- Agitate by stir the jar lightly with your hand, or place on a magnetic stir bar
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Daily Agitation:
- Stir the contents of the jar once daily to promote mixing and aeration. This helps the mycelium grow and distribute evenly throughout the liquid.
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Verify Sterility:
- after 7-10 days you will see thick robust mycelium growth through the jar. Before using the liquid culture, verify its sterility by placing a small amount on agar plates. Ensure that no contaminating organisms are present.
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Using the Colonized Culture:
- Once the jar has fully colonized verify it's sterility again on agar. After confirming its sterility on agar, you can confidently use it on your grain.
Tips:
- Patience is key; We do not refund or replace used jars, do not rush the verification and waiting periods for the best results.
- Always follow good sterile techniques during inoculation and handling to maintain the integrity of your cultures.
By following these instructions, you can ensure a successful and sterile liquid culture expansion for Lion's Mane mushrooms. Enjoy your cultivation journey!
Please note:
Some residual liquid may seep into the bag, which is completely normal and will not compromise the sterility of the jar. Additionally, you may notice some sediments; this is a typical occurrence with the sugar media used.